CAT No.BT-CYT-435 |
Adiponectin Mouse Recombinant, HEK, Acrp30 Mouse, HEK, 10ug |
Amino Acid Sequence
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK.
Applications
ELISA
Western blotting
Cell culture and/or animal studies.
Biological Activity
Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes.
Description
The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.
Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer.
Patent Rights
The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).
Purity
Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed).
Solubility
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Source
HEK293 (Human embryonic kidney cell line).
Stability
Store lyophilized APM-1 at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.