CAT No.BT-CYT-233 |
Adiponectin Human Recombinant, Trimeric form, Acrp30 Human, Trimeric, 10ug |
Amino Acid Sequence
ETTTQGPGVL LPLPKGAATG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK.
Activity
ED50= 3-8.5 ¦Ìg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.
Applications
In vitro, ex vivo and in vivo activity analysis, binding assay et al.
Description
Trimeric form of Adiponectin Human trimeric form was expressed in HEK293 cells. The cysteine 39 was replaced with Alanine (C39A) 9. hAd-C39A can only form trimer, but not hexamer or HMW form.
Formulation
Lyophilized from 0.5 mg/ml in 0.05 M phosphate buffer, 0.05 M NaCl, pH 7.2.
Patent Rights
The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).
Purity
Greater than 95% as determined by SDS-PAGE.
Solubility
Add deionized water to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Source
HEK293 (Human embryonic kidney cell line).
Stability
Store lyophilized Adiponectin at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized AdipoQ remains stable until the expiry date when stored at -20¡ãC.
Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.