Adiponectin Mouse Recombinant, Trimeric form, Acrp30 Mouse, Trimeric, 10ug

Introduction
Adiponectin is a hormone exclusively expressed from adipose tissue. Many studies demonstrate that adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle, attenuate hepatic lipogenesis and luconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells.
In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (MMW, also called trimer) forms 8. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.

Physical appearance
Filtered white lyophilized (freeze-dried) powder.

Activity
ED50= 3-8.5 ¦Ìg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.

Applications
Applications for Acrp30 Mouse include: In vitro, ex vivo and in vivo activity analysis, binding assay et al.

Description
Trimeric form of Acrp30 Mouse was expressed in HEK293 cells.
The cysteine 39 was replaced with alanine (C39A) 9. mAd-C39A can only form trimer, but not hexamer or HMW form.

Formulation
Mouse Acrp30 filtered (0.4?m) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2.

Patent Rights
The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).

Purity
Acrp30 Mouse purity is greater than 95% as determined by SDS-PAGE.

Solubility
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Source
HEK293 (Human embryonic kidney cell line).

Stability
Store lyophilized Acrp30 Mouse at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Mouse can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized Acrp30 Mouse remains stable until the expiry date when stored at -20¡ãC.

Synonyms
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.