| CAT No.BT-ENZ-305 |
| Alanyl-tRNA Synthetase Human Recombinant, PL 12 Human, 20ug |
Description
Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton.
PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.
Formulation
PL-12 is supplied in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Alanyl-tRNA synthetase cytoplasmic, EC 6.1.1.7, Alanine-tRNA ligase, AlaRS, Renal carcinoma antigen NY-REN-42, PL-12, AARS.
