| CAT No.BT-ENZ-276 |
| Disulfide Oxidoreductase Recombinant, DsbA, 50ug |
Amino acid sequence
The sequence of the first five N-terminal amino acids
was determined and was found to be Met-Ly-Lys-Ala-Trp.
Applications
Suitable for use in Western Blot. If this protein is to be used for Western Blot analysis, we recommend that the material be diluted in 1X SDS-PAGE sample buffer (1). On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot with D9625 at a dilution of 1:10,000 as the primary antibody and an appropriate alkaline phosphatase conjugated secondary antibody for detection.
Description
Disulfide Oxidoreductase produced in E.Coli is a periplasmic protein isolated from E. coli, containing 208 amino acids having a molecular mass of 23,149 Dalton.
The DsbA is purified by proprietary chromatographic techniques.
The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized DsbA in sterile 18M¦¸-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution DsbA should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
DsbA, Thiol:disulfide interchange protein dsbA.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
