| CAT No.BT-PRO-400 |
| Calbindin-1 Rat Recombinant, CALB1 Rat, 5ug |
Applications
CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedureA- Dilute 1 ?l of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.
Description
Recombinant Rat Calbindin-1 produced in E.Coli.
The Rat CALB1 is purified by proprietary chromatographic techniques.
Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 0.1mM CaCl2.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized CABP28K in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution CABP28K should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
