| CAT No.BT-HOR-250 |
| Chorionic Gonadotropin Human, hCG, 5mg |
Biological Activity
The activity was found to be 5000 IU/mg.
Contaminents
Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.
Description
Human Chorionic Gonadotropin is produced from a sterile preparation of placental glucoprotein urine of pregnant women having a total molecular mass of 36,700 Dalton. The hCG consists of 237 amino acids, a chain-92 amino acids and b chain-145 amino acids. The hCG is purified by proprietary chromatographic techniques.
The hCG was lyophilized with no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of hCG as a Reference Standard.
Purity
Purity is 98%.
References
1.
Title: Human Chorionic Gonadotropin Modulates Prostate Cancer Cell Survival after Irradiation or HMG CoA Reductase Inhibitor Treatment
Publication: Published online before print October 18, 2006, doi: 10.1124/mol.106.031153 Molecular Pharmacology January 2007 vol. 71 no. 1 259-275
Link:
http://molpharm.aspetjournals.org/content/71/1/259.full
Solubility
It is recommended to reconstitute the lyophilized Human Chorionic Gonadotropin in sterile 18M¦¸-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Source
Urine of pregnant women.
Stability
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution CG-beta should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Chorionic gonadotropin beta polypeptide, CGB3, hCGB, Choriogonadotropin subunit beta, CG-beta, Chorionic gonadotrophin chain beta.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
