| CAT No.BT-ENZ-311 |
| Enteropeptidase/Enterokinase Light Chain Bovine Recombinant, Enterokinase Bovine, 100IU |
Description
Enterokinase (rEK) Bovine Recombinant is the catalytic subunit of bovine enterokinase, which is expressed by the yeast and purified to yield a high enzyme activity preparation. EK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence.
Recombinant Bovine Enterokinase is a single glycosylated polypeptide chain containing 235 amino acids and having an MW of ~43kDa.
Assay Conditions
Each reaction contains 50 ug of partially purified thioredoxin-NP-27 fusion protein and varying amounts of Bovine EK. Reactions were incubated at 25¡ãC for 16 hours and analyzed on a Coomassiestained 15% SDS gel.
Formulation
Bovine EK in 50mM Tris-HCl, pH 8.0, 0.5M NaCl and 50% glycerol.
Sterile liquid solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Source
Pichia pastoris.
Stability
One year when stored at ¨C20¡ãC.
Please avoid freeze-thaw cycles.
Synonyms
Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK, MGC133046.
Unit Defenition
One unit is defined as the amount of enzyme needed to cleave 50ug of fusion protein in 16 hours to 95% completion at 25¡ãC in a buffer containing 25mM Tris-HCl, pH 7.6, 50mM NaCl, and 2mM CaCl2.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
