Enteropeptidase/Enterokinase, Light Chain Human Recombinant, Enterokinase Human, 100IU

Assay Conditions
50mM Tris-HCl or sodium phosphate (pH 8.0) at 25¡ãC with or without CaCl2. The enzyme is active at a pH range of 6.0-9.0.

Description
Enteropeptidase Human is a specific protease that cleaves after the sequence
Asp-Asp-Aps-Aps-Lys. The light chain of enteropeptidase has full enzymatic activity. No other protease activity was detected. Human enteropeptidase binds specifically to STI-agarose. The affinity puirifed Human Enterokinase contains amino acids from 785 to 1019.

Dilution buffer
50mM Tris-Hcl, pH 8.0 at 25C, 500mM NaCl, 1mM CaCl2 and 50% glycerol.

Physical Appearance
Liquid solution.

Purity
Greater than 98.0% as determined by SDS-PAGE.

Source
Escherichia Coli.

Stability
One year when stored at ¨C20¡ãC, three weeks at room temperature.

Synonyms
Enteropeptidase, EC 3.4.21.9, Enterokinase, Serine protease 7, ENTK,TMPRSS15, MGC133046, Transmembrane Protease Serine 15.

Unit Defenition
One unit of human enteropeptidase will cleave 2 mg of thioredoxin/human EGF fusion protein with the Asp-Asp-Asp-Asp-Lys sequence at the joining point in 22 hours at 4¡ãC, in 16 hours at 25¡ãC or in 8 hours at 37¡ãC.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.