| CAT No.BT-ENZ-289 |
| Isocitrate Dehydrogenase (NADP +) Recombinant, iCDH, 5mg |
Biological Activity
The specific activity was found to be 119 U/mg.
Description
Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
The ICDH is purified by proprietary chromatographic techniques.
Formulation
One ml of solution (1mg/340?l) contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Source
Saccharomyces Cerevisiae.
Stability
ICDH although stable at 15¡ãC for 1 week should be stored between
2¡ãC-8¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA)
Please avoid freeze-thaw cycles.
Synonyms
Isocitrate dehydrogenase [NADP] cytoplasmic, EC 1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase, Oxalosuccinate decarboxylase, IDH, NADP(+)-specific ICDH, IDP, PICD.
Unit Definition
One unit is defined as 1 ?mol of NAD+ production per minute under the assay conditions (25¡ãC, pH 7.5).
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
