| CAT No.BT-ENZ-327 |
| Secreted Phospholipase A2-IIE Human Recombinant, PLA2G2E Human, 10ug |
Applications
Western blotting.
Description
Secreted Phospholipase A2-IIE Human Recombinant manufactured with N-terminal His-Tag. PLA2G2E His-Tagged Fusion Protein is 15.8 kDa protein containing 123 amino acid residues of the human secreted phospholipase A2-IIE and 16 additional amino acid residues ¨C His-Tag (underlined).
MRGSHHHHHHGMASHMNLVQFGVMIEKMTGKSALQYNDYGCYCGIGGSH
WPVDQTDWCCHAHDCCYGRLEKLGCEPKLEKYLFSVSERGIFCAGRTTCQR
LTCECDKRAALCFRRNLGTYNRKYAHYPNKLCTGPTPPC.
Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Sterile Filtered lyophilized (freeze-dried) powder.
Purification Method
Ni-NTA affinity chromatography.
Purity
Greater than 95% as determined by SDS PAGE.
Solubility
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.
Source
Escherichia Coli.
Specificity
The amino acid sequence of the recombinant human Secreted Phospholipase A2-IIE is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-IIE without signal sequence.
Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.
Synonyms
Group IIE secretory phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase GIIE, GIIE sPLA2, sPLA(2)-IIE, sPLA2-IIE, PLA2G2E.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
