| CAT No.BT-CYT-439 |
| Macrophage-Colony Stimulating Factor Mouse Recombinant, M CSF Mouse, 10ug |
Amino acid sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.
Biological Activity
The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found 0.91ng/ml, corresponding to a specific activity of 1MIU/mg.
Description
Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.
The protein (1mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized M-CSF in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution MCSF should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
Please prevent freeze-thaw cycles.
Synonyms
CSF-1, Lanimostim, MCSF, M-CSF.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
