| CAT No.BT-PRO-446 |
| Modified Affinity Purified Avidin, Avidin Biotin, 10mg |
Activity
Binding activity of Avidin-Biotin is 10-15units/mg protein.
One unit of Avidin activity is defined as the amount of protein which will bind 1ug d-biotin.
Advantages
1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.
2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin.
3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.
Applications
Avidin-Biotin may be used in combination with biotin in various techniques such as ELISA, Western blotting, immunohistochemistry etc¡
Physical AppearanceLyophilized freeze dry Powder.
Avidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons.
Avidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.
Formulation
Salt-free lyophilized powder with no preservative added.
Solubility
We highly recommended to prepare a 1 mg/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.
Stability
Store protein solution at 2-8¡ãC.
Synonyms
Avidin, AVD, AVID.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
