| CAT No.BT-ENZ-309 |
| Taq Plus DNA Polymerase Recombinant, Taq Plus DNA, 750U |
10X Reaction Buffer
200 mM TrisHCI (pH 8.8)
100 mM KCI
100 mM (NH4)2SO4
20 mM Mg SO4
1% Triton X-100
1 mg/ml bovine serum albumin (BSA).
Concentration
5U/?l.
Description
Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78¡ãC and remains > 95% active following 1-hour incubation at 95¡ãC.
All reagents, including Taq Plus, should be mixed immediately before use.
Optimization of DNA Synthesis
It is important to ad the reaction components in the following order1. H2O.
2. 10 x reaction buffer.
3. dNTPs.
4. DNA template and primers.
5. Taq Plus.
Reaction Conditions
DNA synthesis is performed in 100?l of mixture containing 20-200¦Ìm dNTPs, 0.3-1¦Ìm Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA.
Source
Recombinant E.coli contains Thermus aquaticus polymerase gene.
Storage Buffer
20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.
Storage Conditions
Stable for 5 days at 10¡ãC, for longer period of time store at -20¡ãC.
Synonyms
Taq Plus DNA Polymerase, Taq Plus DNA, TaqPDNA.
Unit Definition
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74¡ãC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
