| CAT No.BT-ENZ-285 |
| Thyroid Peroxidase Human Recombinant, TPO Human, 20ug |
Applications
Western-Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody.
Coating concentration
0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.
Description
Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.
The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Auto-antibodies to TPO recognize conformation-dependent epitopes.
3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).
Purity
Greater than 95% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
