| CAT No.BT-ENZ-263 |
| Tissue Plasminogen Activator Human Recombinant, tPA Human, 100ug |
Description
Tissue Plasminogen Activator Human Recombinant produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin.
The tPA is purified by proprietary chromatographic techniques.
Enzymatic Activity
580,000 IU/mg.
Formulation
Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized t-PA in sterile 18M¦¸-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Source
Chinese Hamster Ovary Cells (CHO)
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized t-PA although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution tPA should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
