| CAT No.BT-ENZ-303 |
| Tissue Transglutaminase Human Recombinant, Sf9, TGM2 Human, Sf9, 20ug |
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody.
Coating concentration
0.6-1.4 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Description
Tissue Transglutaminase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78,018 Dalton. tTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.
TGM2 is supplied in 16mM HEPES buffer pH-8.0, 320mM NaCl, and 20% glycerol.
Immunological functions
1. Binds IgA & IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
Purity
Greater than 95% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Protein-glutamine gamma-glutamyltransferase 2, EC 2.3.2.13, Tissue transglutaminase, TGase C, TGC, TG(C), Transglutaminase-2, TGase-H, TG2, TGM2.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
