| CAT No.BT-ENZ-344 |
| Ubiquitin-Conjugating Enzyme E2L 3 Human Recombinant, His Tag, UBE2L3 Human His, 50ug |
Amino acid sequence
MHHHHHHAMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVP
DNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAEN
WKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFT
KKYGEKRPVD.
Description
Ubiquitin-Conjugating Enzyme E2L 3 Human Recombinant produced in E.coli is an 18.9 kDa protein containing 162 amino acids.
The UBE2L3 protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Formulation
Lyophilized from a 0.2¦Ìm filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Sterile Filtered white lyophilized powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized UBE2L3 in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized UBE2L3 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution UBE2L3 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Ubiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3,Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, UbcM4.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
