| CAT No.BT-ENZ-274 |
| Ubiquitin-Conjugating Enzyme E2I Human Recombinant, His Tag, UBE2I Human His, 50ug |
Amino acid sequence
MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGT
MNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFH
PNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTI
YCQNRVEYEKRVRAQAKKFAPS.
Description
Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E.coli is a 19.5 kDa protein containing 171 amino acids.
The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Formulation
Lyophilized from a 0.2¦Ìm filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Sterile Filtered white lyophilized powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution UBE2I should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
