| CAT No.BT-ENZ-345 |
| Ubiquitin Conjugating Enzyme E2M Human Recombinant, UBE2M Human, 50ug |
Amino acid sequence
MSYYHHHHHHDYDIPTTENLYFQGAMDPEFRIWMIKLFSLKQQKKEEE
SAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVIC
PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLN
ILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAVLQNNRRLFEQNVQ
RSMRGGYIGSTYFERCLK.
Description
Ubiquitin Conjugating Enzyme E2M Human Recombinant produced in E.coli is a 25 kDa protein containing 216 amino acids.
The UBE2M protein contains 6xHis tag and is purified by proprietary chromatographic techniques.
Formulation
Lyophilized from a 0.2¦Ìm filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Sterile Filtered whilte lyophilized powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized UBE2M in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized UBE2M although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution UBE2M should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
NEDD8-conjugating enzyme Ubc12, Ubiquitin-conjugating enzyme E2 M, NEDD8 protein ligase, NEDD8 carrier protein, UBC12, hUbc12, UBC-RS2.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
