| CAT No.BT-ENZ-352 |
| Uracil DNA Glycosilase, UNG, 10,000U |
10X UNG Reaction Buffer
200mM Tris-HCl (pH 8.0 at 25¡ãC), 10mM DTT and 10mM EDTA.
Applications
Glycosilase mediated single nucleotide polymorphism detection (GMPD).
Site-directed mutagenesis.
As a probe for protein-DNA interaction studies.
Rapid and efficient cloning of PCR products.
Elimination carry-over contamination in PCR.
Description
E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
UNG solution in 10mM Tris-Hcl (pH-7.4 at 25¡ãC), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Inhibition and Inactivation
Inactivated by heating at 95¡ãC for 10min. Enzye activity is partially restored at temperatures lower than 55¡ãC.
Physical Appearance
Sterile Filtered colorless solution.
Reaction Conditions
1X UNG Reaction Buffer, incubate at 37¡ãC.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Source
Escherichia Coli strain that carries the UNG gene from E.coli.
Specific Activity
The Specific Activity was found to be 5U/?l.
Stability
Uracil DNA Glycosilase although stable at 15¡ãC for 1 week, should be stored desiccated below -18¡ãC.
Please prevent freeze-thaw cycles.
Synonyms
Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.
Unit Definition
1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37¡ãC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
