Vascular Endothelial Growth Factor Human Recombinant, CHO, VEGF Human, CHO, 10ug

Biological Activity
Determined by its ability to stimulate 3H-Thymidine incorporation in human ublical vein endothelial cells, the ED50 for this effect was found to be 2-6ng/ml corresponding to a Specific Activity of 166,667-500,000IU/mg.

Description
Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions.
The VEGF is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Chinese Hamster Ovarian Cell.

Stability
Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution VEGF Human Recombinant should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.